The HTEC-510 is user friendly, provides worry free maintenance, can be easily switched between methods, and most importantly has superb sensitivity.
Ultra-High Sensitivity Electrochemical Detection
The best advantage of using electrochemical (EC) detection is the sensitivity. Eicom will never compromise when improving the electrochemical detector’s sensitivity. The combination of the HTEC-510, HPLC-Electrochemical Detector and the appropriate applications will easily ensure 160 amol sensitivity for dopamine and serotonin, and 5 fmol for acetylcholine.
Turn-Key System
The HTEC-510 is the optimized HPLC-ECD (Electrochemical Detection) system. Analytical applications of electrochemical detection have already been prepared, all you need to do is choose the appropriate method and follow the detailed protocol. Then you can easily get the results.
Easy to Maintain
The simple compact design of the HTEC-510 (HPLC-ECD) allows for easy maintenance. If you have any questions, we will be happy to help you every step of the way.
Superior Customer Support
We assign a trained application specialist to work directly with you from the beginning. This way we build an efficient working relationship and can help resolve any issues immediately.
The HTEC-510 replaces the HTEC-500
Eicom’s Innovative Cell Design
- Ultrasensitive, 30 fg of dopamine
- Graphite electrode often ready to use < 30 mins after start-up
- No tools to access the working electrode
- Easy to clean or change the working electrode
The key to the sensitivity is the cell design. Eicom relies on a spring loaded mechanism that always maintains the proper pressure. This structure allows the detector to quickly stabilize after the cell is opened and closed. Another benefit is that the working electrode can easily be accessed without any tools. In this way, the electrode can easily be wiped clean and reinstalled to insure maximum sensitivity.
System
| System | The degasser, pump and ECD are mounted in a temperature controlled cabinet. A manual injector is also included. |
|---|---|
| System Control | Integrated central control |
| Materials in Contact With Solvents | PEEK and FEP, metal free materials |
| Flow Line Pressure Resistance | 20 MPa |
| Detector (1st Channel) | Amperometric DC mode |
| Detector (2nd Channel) Option | Coulometric ECD or UV/Vis detection |
| Dimensions | 250 (w) x 399 (h) x 365 (d) mm/approx 10″ x 16″ x 14.5″ |
| Weight | 18 kg with amperometric detector cell/40 lb |
Electrochemical Detection (1st channel)
| Electrochemical Detection (1st channel) | Expandable for second channel with model EPC-510 |
|---|---|
| Principle | Amperometric DC mode |
| Applied Potential Range | 0 to +/- 2000 mV, 1 mV steps |
| Integrator Output Range | Auto-zero point +/- 5 V, 0.1 nA/1 mV |
| Auto-Zero Working Range | +/- 500 nA |
| Detector Cell | Easy open/close with constant pressure system |
| Working Electrode | Exchangeable disk |
| Standard Working Electrode | Graphite, S/N 250% improved from glassy carbon |
| Working Electrode Options | Platinum, modified gold, pure graphite, glassy carbon, silver |
| Surface Volume of Working Electrode | Changeable, 25 or 50 µm thickness with 3.5 or 7 mm width |
| Reference Electrode Material | Ag/AgCl, solid phase |
| Counter Electrode Material | Stainless steel 316 |
| Time Constant Filter | 1.0/1.5/3 min |
| Noise Level | Less than 3 pA with dummy flow of R-W 1 MΩ R-C 2 MΩ at 0 mV |
High Performance Pump
| High Performance Pump | |
|---|---|
| Single Stroke Replacement Volume | 4 µl, Φ2 x1.3 mm stroke |
| Principle | Isocratic, reciprocal dual piston pump |
| Pulsation Control | Controllable on/off capability. Computer programmed auto-quenching pulse system by variable stroke speed drive during each stroke. |
| Pistons Material | Sapphire glass |
| Check Valves | Φ1/16″ two check valves at the outlet and a single valve at the inlet |
| Piton Seal | High density polyethylene, Teflon® also available |
| Flow Rate Range | 0 to 750 µl/min |
| Pulse Damper | Not required |
| Error Monitor | Over pressure limit error, check valve error, motor error |
Thermoregulation
| Thermoregulation | |
|---|---|
| Temperature Control Range | 20-45°C at 25°C ambient |
| Control Procedure | Peltier element to both cool and heat |
Solvent Degassing Unit
| Solvent Degassing Unit | |
|---|---|
| Principle | Online |
| Channel | Two |
| Internal Volume | Ch 1 300 µl/Ch 2 7.5 ml |
Communication
| Communication | All connectors are easy to access outside the component |
|---|---|
| ECD Integrator Out | Analog, 0.1 nA = 1 mV, 10 V full range |
| Pump Pressure Monitor Out | Analog, 10 MPa = 100 mV |
| Temperature Monitor Out | Analog 10°C = 100 mV. |
| Pump Error Out | 1.0 sec contact closure signal out. Works to stop autosampler. |
| Stop/Run Outer Trigger In | Stop/run ECD and pump unit. 0.5 sec contact closure signal in. Works for auto stop and auto start. Main power required to be on to receive the signal. |
| Auto-Zero Trigger In | Runs auto-zero, 0.5 sec contact closure signal in |
| Manual Injector Switching Signal In | After receiving the signal, the delay out sends signal out. |
| Inject Signal In | After receiving the signal, the delay out sends signal out. Works to communicate with an autosampler. |
| Delay Out | 2 ports. 0 and 3 sec delay with 1.0 sec contact closure signal. |
The specifications are subject to change without notice.
