Eicom’s ENO-30 is a unique NOx analyzer developed for biological liquid samples.

Streamline Your Nitrite and Nitrate Analysis

  • Suitable for all biological fluids (1-50 µL injections)
  • Single injection to measure both nitrite and nitrate
  • Sensitivity is better than 0.1pmol (10 nM for 10 µL)
  • Easy sample preparation >Sample types and preparation
  • Capability for fully automated analyses with autosampler

The ENO-30 continues the tradition of Eicom’s successful ENO-20 NOx analyzer. The ENO-30 system is a specialized HPLC for separating and detecting nitrite and nitrate in various biological fluids. The system makes use of a simple Griess reaction, in which nitrite reacts to form a light absorbing compound that can easily be detected.  However, compared to a typical plate or tube based Griess assay, the ENO-30 greatly enhances sensitivity and reproducibility.  In addition, high throughput analysis is achieved by pairing the instrument with an HPLC autosampler, an impossible feat for the gas phase chemiluminescent detectors sometimes used to detect nitric oxide. Data collection, calibration, and analysis are handled by Eicom’s Envision software package.

Sample Data of ENO-30 NOx Analyzer


The data shown here is a typical profile obtained from a human plasma sample using Eicom ENO-30, NOx Analyzer. The peaks correspond to 110 nM nitrite and 73 µM nitrate in 10 µl applied sample to the ENO-30.

Perfect for Biological Samples

Sensitive enough for small volume biological samples. The typical sample is just 1-50 μL of blood plasma, urine, saliva, breathe condensate, tissue homogenate, cell culture supernatant, or microdialysate. Simple sample preparation consist of the addition of an equal volume of methanol to inactive and precipitate proteins, whereas low protein samples can be direct injected without prior manipulation.

How does it work?

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Pump Unit

Pump Unit
TypeTwo independent dual reciprocating pumps
Flow RateEach settable ~ 1 – 750 µl/min
Carrier: 0.33 mL/min
Reactor: 0.10 mL/min
Stroke4 µl replacement volume
Pulse Damping FunctionActively, computer-controlled, based on pressure feedback mechanism
Maximum Pressure20 Mpa
Pressure LimitSettable between 0-20 Mpa (error 1 triggered)
Piston MaterialSapphire
Other Liquid Contacting SurfacesPEEK, Sapphire, Ruby, PTFE, PCTFE
DegasserTwo 300 µL loops
Size400 (w) x 400 (d) x 190 (h) mm
PowerAC100-240V 50/60Hz, 200W
Weight~16 kg

Detector/Oven Unit

Detector/Oven Unit
Detection PrincipleLight absorption at 540 nm (diazo compound)
Detection Limit10 nM x 10 µL (0.1 pmol)
Temperature Control15°C – 45°C at room temp of 25°C
Temperature Accuracy± 0.1°C
Size400 (w) x 400 (d) x 145 (h) mm
PowerAC100-240V 50/60Hz, 200W
Weight~11 kg

The specifications are subject to change without notice.

For more product details visit NOx Analyzer Details
See also Nitrite/Nitrate Physiological Role